Andor Spinning Disk

06.07.2022

High Speed Confocal Microscope System | Dragonfly.
Andor TIRF & Spinning Disk Confocal | Whitehead Institute.
Andor XD Spinning Disk Confocal Microscope Manual.
Andor Dragonfly Spinning Disk Confocal Microscope | USF Health.
Andor Spinning Disk Confocal Microscope - Wisconsin Centers for.
Andor Dragonfly Spinning Disk Confocal plus - Duke University.
PDF Andor/Olympus Spinning Disk User Guide.
Andor Spinning Disk Confocal Microscope - Brandeis University.
Spinning Disk Confocal (Andor/Olympus) | Cornell Institute of.
An Overview of Spinning Disk Confocal Microscopy.
Benefits of confocal spinning disk microscopy and Andor's Dragonfly.
Andor Dragonfly Spinning Disk Confocal Microscope (User's Manual).
Confocal Dual Spinning Disk - Andor - Oxford Instruments.
Andor XD Revolution Spinning Disk Confocal | Light Microscopy.

High Speed Confocal Microscope System | Dragonfly.

This technology improvement, in combination with using an electron multiplying CCD detector, results in spinning disk technology being the ideal solution for fast live cell confocal imaging. Figure 2 shows how the dual spinning disk confocal laser scanner operates. Unlike a conventional laser-scanning microscope, where a narrow laser beam sequentially scans the sample, in. Benefits of confocal spinning disk microscopy and Andor's Dragonfly. Dr Peter March moved to Manchester for his Biochemistry degree at the University of Manchester and after his PhD, become the resident microscopist. At the time, facility management was not a common job. Nevertheless, Peter started as the "Microscopy Facility Manager" at.

Andor TIRF & Spinning Disk Confocal | Whitehead Institute.

Andor Dragonfly Spinning Disk Confocal Microscope (User's Manual) Overview High-speed and high-sensitivity multi-modal confocal imaging with 2 pinhole sizes (25um and 40um) Four laser lines: 405nm, 488nm, 561nm, and 638nm Borealis Uniform Illumination Environmental control for samples which need heating and CO2. Andor Revolution X1 Spinning Disk confocal inverted microscope. * Andor 4 laser stack (405, 488, 561, 640 nm). * Olympus IX81 inverted microscope * Yokogawa CSU-X1 spinning disk scanhead CSUX1-M1N... M1 = basic model (no widefield bypass), N = normal standard speed (5000rpm). * MetaMorph acquisition..

Andor XD Spinning Disk Confocal Microscope Manual.

Andor Dragonfly Spinning Disk Confocal Microscope | USF Health.

Spinning disk confocal scan head. Nikon. (link is external) Ti-E invertedmicroscope stand with Perfect Focus System, motorized stage and DIC and phase optics. Prior. (link is external) Lumen200 metal halide light source. 4 laser lines in Andor ALC: 405nm, 488nm, 561nm, and 642nm.

Andor Spinning Disk Confocal Microscope - Wisconsin Centers for.

In the Andor implementation of the spinning disk, the laser light which enters parallel is focused through an array of micro-lens for each pinhole to ensure good coupling efficiency. The patented array of microlens allows very efficient coupling of the laser to the pinholes which reduces the power of laser needed to achieve good confocal imagines. Andor XD Spinning Disk Confocal Microscope Manual Location LSRC C142 Capabilities High-speed multidimensional imaging with optical sectioning Spinning disk modality produces high SNR images with minimal photobleaching High sensitivity EMCCD camera Laser lines for all classes of fluorscent proteins Incubation for mammalian cells.

Andor Dragonfly Spinning Disk Confocal plus - Duke University.

5 Multi Dimensional Acquisition (MDA) For acquiring Z-series or multiple positions or multiple colors, the MDA window can be accessed via the Multi Dimensional Acquisition button on the task bar. 1) Check the appropriate boxes for the type of imaging acquisition that is required. The Dragonfly naturally benefits from Andor EMCCD and sCMOS technologies, with motorized optical zoom, the system delivers industry-leading signal to noise and image fidelity. Applications ranging from single molecule to live cell confocal, TIRFM to whole embryo and thick tissue imaging all benefit from the system’s speed and sensitivity. Nikon-Andor Spinning Disk. Motorized, inverted Nikon Ti-E microscope with Perfect Focus System equipped with Yokogawa CSU-X1 spinning disk and Andor Laser launcher with 405, 491, 514, and 561 nm laser lines. The system is equipped with 20x NA 0.5, 40x NA 0.75, 60x NA 1.4 oil and 100 NA 1.4 oil objectives. Each pinhole has a diameter of 50µm, being.

PDF Andor/Olympus Spinning Disk User Guide.

Andor Spinning Disk/TIRF/Widefield Overview: This is a multi-modal microscope configured around the Andor Revolution Spinning Disk confocal system. The Yokogawa spinning disk and frame transfer camera are optimized for fast time lapse. The Andor Spinning Disk confocal is an optical imaging technique with increased resolution as compared to widefield microscopy. Higher scanning speeds are possible with spinning disk confocal, decreasing the potential for sample photobleaching and phototoxicity. Andor Dragonfly Spinning Disk Confocal Microscope. The Dragonfly system is a versatile multi-dimensional imaging platform offering three key modalities; high-speed/sensitivity spinning disk confocal imaging, laser-illuminated widefield epifluorescence, and high contrast TIRF (total internal reflectance) microscopy.

Andor Spinning Disk Confocal Microscope - Brandeis University.

The Andor Spinning Disk Confocal Microscope, located on the 5th floor of the Rosenstiel Center, is equipped with both spinning disk confocal and epifluorescence modes, as well as a Mosaic laser photobleaching / photoactivation device. The system is set up to maximize speed for sequential or simultaneous detection of multiple fluorophores and.

Spinning Disk Confocal (Andor/Olympus) | Cornell Institute of.

The Andor Dragonfly 302 Spinning disk confocal is attached to a Nikon Eclipse Ti microscope and has a range of oil immersion objectives and TIRF (Total Internal Reflection Fluorescence) capability. The system is optimized for resolution and has two cameras: Zyla 4.2 Megapixel sCMOS camera and iXon Ultra 888 Ultra EM CCD Camera (1024 x 1024 pixels). Scanhead. Andor Dragonfly 505 unit with Borealis illumination spinning disk confocal - 2 pinhole sizes (25 um and 40 um) TIRF microscopy via a separate lightpath. Motorized dichroics - BGR (405/488/561/640) and CY (445/515) Additional technology on system: TIRF microscopy via a separate lightpath. Facilities (BRC) Instruments Spinning Disk Confocal (Andor/Olympus) Reserve instrument Request training Get a 50% discount for 8 or more continuous off-peak evening hours Inverted Olympus IX-83 microscope DIC optics, high NA immersion objective lenses 405, 488, 561, and 640 nm laser lines Multiple cameras for truly simultaneous color imaging.

An Overview of Spinning Disk Confocal Microscopy.

The Andor Revolution XD system is a spinning disk laser confocal microscope with a sensitive EMCCD camera suitable for fast in vivo imaging. It's the fastest and most sensitive system at the institute. The system can also scan multiple positions in the sample, with multiple channels in timelapse mode. Andor Dragonfly IMB Imaging Facility Page 1 Andor Dragonfly Spinning Disk Quickstart Guide Location: Room 6.025B (Updated: 22/8/2019) Acquisition Software: Fusion Visualisation Software: Imaris Available Laser Lines: 405, 445, 488, 514, 561 and 637nm Available Objective Lenses: 4x Air, 10x Air, 20x Air, 40x Air, 40x Water, 60x Oil and 100x TIRF Oil.